• Training Period: 2011-2012, MSc 2011-2016, PhD
  • Prior Institution: Adam Mickiewicz University, Poland
  • Prior Degree: MSc (2011)
  • Project Title: Structural and functional studies of serum albumins.
  • Current Position: Broad Institute, Boston, Massachusets, USA
Education:
  • M.Sc in Bioinformatics - Adam Mickiewicz University - Poznan, Poland - 2009-2011
  • B.Sc in Bioinformatics - Adam Mickiewicz University - Poznan, Poland - 2006-2009
    • Research Interest:
  • Serum albumin (SA) as a carrier protein - interaction of SA with metabolites and drugs
  • Metal binding to proteins and RNA with the focus on metal interaction with serum albumin and Mg-binding sites validation and classification in RNA crystal structures
  • Protocol development and optimization of macromolecular crystallization experiments
  • Protein structure determination and refinement
    • Projects:
    My thesis research focuses on studying the interaction between serum albumin (SA) and selected metabolites and drugs on the molecular and biochemical levels. I am particularly interested in determining molecular basis of metals interaction with SA, as well as how the physiologic changes in pH affect metal binding and SA structure. The principal technique is X-ray crystallography.
    CV:
  • “Circulatory zinc transport is controlled by distinct interdomain sites on mammalian albumins.” – Highlighted article on the inside back cover of Chemical Science (Issue 11)
  • Graduated with Honors for Doctorate Research - Polish Academy of Science (2016)
  • Margaret C. Etter Student Lecture Award - American Crystallographic Association (ACA) Annual Meeting, Philadelphia, PA (2015)

    • Publications with the Minor Lab

    1. Czub MP, Handing KB, Venkataramany BS, Cooper DR, Shabalin IG, Minor W (2020)
      Albumin-Based Transport of Nonsteroidal Anti-Inflammatory Drugs in Mammalian Blood Plasma.
      J Med Chem 63: 6847-6862. [DOI:10.1021/acs.jmedchem.0c00225] [Pub Med ID: 32469516] [Pub Med Central ID: PMC7902233] Times cited: 10
    2. Czub MP, Venkataramany BS, Majorek KA, Handing KB, Porebski PJ, Beeram SR, Suh K, Woolfork AG, Hage DS, Shabalin IG, Minor W (2019)
      Testosterone meets albumin - the molecular mechanism of sex hormone transport by serum albumins.
      Chem Sci 10: 1607-1618. [DOI:10.1039/c8sc04397c] [Pub Med ID: 30842823] [Pub Med Central ID: PMC6371759] Times cited: 17
    3. Handing KB, Niedzialkowska E, Shabalin IG, Kuhn ML, Zheng H, Minor W (2018)
      Characterizing metal-binding sites in proteins with X-ray crystallography.
      Nat Protoc 13: 1062-1090. [DOI:10.1038/nprot.2018.018] [Pub Med ID: 29674755] [Pub Med Central ID: PMC6235626] Times cited: 30
      F1000 Recommended Publication
    4. Kutner J, Shabalin IG, Matelska D, Handing KB, Gasiorowska O, Sroka P, Gorna MW, Ginalski K, Wozniak K, Minor W (2018)
      Structural, Biochemical, and Evolutionary Characterizations of Glyoxylate/Hydroxypyruvate Reductases Show Their Division into Two Distinct Subfamilies.
      Biochemistry 57: 963-977. [DOI:10.1021/acs.biochem.7b01137] [Pub Med ID: 29309127] [Pub Med Central ID: PMC6469932] Times cited: 10
    5. Zheng H, Cooper DR, Porebski PJ, Shabalin IG, Handing KB, Minor W (2017)
      CheckMyMetal: a macromolecular metal-binding validation tool.
      Acta Crystallogr D Struct Biol 73: 223-233. [DOI:10.1107/S2059798317001061] [Pub Med ID: 28291757] [Pub Med Central ID: PMC5349434] Times cited: 135
    6. Handing KB, Shabalin IG, Kassaar O, Khazaipoul S, Blindauer CA, Stewart AJ, Chruszcz M, Minor W (2016)
      Circulatory zinc transport is controlled by distinct interdomain sites on mammalian albumins.
      Chem Sci 7: 6635-6648. [DOI:10.1039/c6sc02267g] [Pub Med ID: 28567254] [Pub Med Central ID: PMC5450522] Times cited: 39
    7. Handing KB, Shabalin IG, Szlachta K, Majorek KA, Minor W (2016)
      Crystal structure of equine serum albumin in complex with cetirizine reveals a novel drug binding site.
      Mol. Immunol. 71: 143-151. [DOI:10.1016/j.molimm.2016.02.003] [Pub Med ID: 26896718] [Pub Med Central ID: PMC4800003] Times cited: 17
    8. Niedzialkowska E, Gasiorowska O, Handing KB, Majorek KA, Porebski PJ, Shabalin IG, Zasadzinska E, Cymborowski M, Minor W (2016)
      Protein purification and crystallization artifacts: The tale usually not told.
      Protein Sci 25: 720-33. [DOI:10.1002/pro.2861] [Pub Med ID: 26660914] [Pub Med Central ID: PMC4815408] Times cited: 26
    9. Zheng H, Shabalin IG, Handing KB, Bujnicki JM, Minor W (2015)
      Magnesium-binding architectures in RNA crystal structures: validation, binding preferences, classification and motif detection.
      Nucleic Acids Res 43: 3789-801. [DOI:10.1093/nar/gkv225] [Pub Med ID: 25800744] [Pub Med Central ID: PMC4402538] Times cited: 50
    10. Zheng H, Handing KB, Zimmerman MD, Shabalin IG, Almo SC, Minor W (2015)
      X-ray crystallography over the past decade for novel drug discovery - where are we heading next?
      Expert Opin Drug Discov 10: 975-89. [DOI:10.1517/17460441.2015.1061991] [Pub Med ID: 26177814] [Pub Med Central ID: PMC4655606] Times cited: 34
    11. Chruszcz M, Mikolajczak K, Mank N, Majorek KA, Porebski PJ, Minor W (2013)
      Serum albumins-unusual allergens.
      Biochim. Biophys. Acta 1830: 5375-81. [DOI:10.1016/j.bbagen.2013.06.016] [Pub Med ID: 23811341] [Pub Med Central ID: PMC4419372] Times cited: 67