• Graduate Certificate in Leadership, University of Virginia (2016-2019)
  • Ph.D. in Chemistry - A.N. Bach Institute of Biochemistry, Russian Academy of Sciences (2006-2010)
  • M.Sc. - Moscow State University (2001-2006)
Research Interest:
  • Development of novel antimicrobials to cope with antibiotic resistance
  • High-throughput structure determination and functional studies of proteins (including potential drug-targets) from pathogens causing emerging and re-emerging infectious diseases using a combination of X-ray crystallography and other biophysical methods.
  • Developing integrative techniques for highly efficient protein crystallization with a goal of maximizing the number of successful crystallizations, yet minimizing the resources used.
  • Macromolecular structure elucidation and refinement. Exploration of novel methods for crystallographic structure solution and solving "mission impossible" structures.
  • Quality assessment of crystallographic protein structures. Ligand and metal identification and judicious modeling.
  • Metal binding to protein and RNA. Developing a protocol for characterizing metal binding sites in proteins with X-ray crystallography. Developing a protocol for Mg-binding sites validation and classification in RNA crystal structures.
  • Published 25 peer-reviewed publications and 2 book chapters
  • Deposited more than 120 protein structures to the PDB
  • Presentated at 24 conferences and workshops, including 8 invited talks
  • Taught a 5-day course “Structure determination with HKL3000” at RapiData – a practical course in macromolecular X-ray diffraction data collection, data processing and structure solution, Stanford University (2017, 2018, and 2019)
  • Lectured on data processing with HKL3000, structure refinement and validation at Workshop on data collection and structure solving in macromolecular X-ray diffraction. Krakow, Poland (2019)
  • Co-developed an integrative protocol for highly efficient protein crystallization. Achieved 45% success rate for a set of 95 proteins (structure determined for 46 proteins)
  • Co-developed an integrative protocol for characterizing metal-binding sites in proteins with X-ray crystallography
  • Recipient of the Best Postdoc Poster Award at 43rd Mid-Atlantic Macromolecular Crystallography Meeting (2013)
  • Four awards during graduate/undergraduate studies
  • Contributed to the development of MgRNA server
  • Contributed to the development of CheckMyMetal server
  • Contributed to the development of 2HADH knowledgebase

Google Scholar Profile


Publications with the Minor Lab

  1. Lipowska J, Miks CD, Kwon K, Shuvalova L, Zheng H, Lewiński K, Cooper DR, Shabalin IG, Minor W (2019)
    Pyrimidine biosynthesis in pathogens - Structures and analysis of dihydroorotases from Yersinia pestis and Vibrio cholerae.
    Int. J. Biol. Macromol. 136: 1176-1187 [Pub Med ID: 31207330] [Pub Med Central ID: PMC6686667]
  2. Czub MP, Venkataramany BS, Majorek KA, Handing KB, Porebski PJ, Beeram SR, Suh K, Woolfork AG, Hage DS, Shabalin IG, Minor W (2019)
    Testosterone meets albumin - the molecular mechanism of sex hormone transport by serum albumins.
    Chem Sci 10: 1607-1618 Times cited: 1 [Pub Med ID: 30842823] [Pub Med Central ID: PMC6371759]
  3. Kowiel M, Brzezinski D, Porebski PJ, Shabalin IG, Jaskolski M, Minor W (2019)
    Automatic recognition of ligands in electron density by machine learning.
    Bioinformatics 35: 452-461 Times cited: 2 [Pub Med ID: 30016407] [Pub Med Central ID: PMC6361236]
  4. Grabowski M, Shabalin I, Porebski PJ, Domagalski MJ, Zheng H, Cooper D, Venkataramany BS, Bourne PE, Minor W (2019)
    Data Sharing in Structural Biology: Advances and Challenges
    in Data Sharing: Recent Progress and Remaining Challenges - Computer Science, Technology and Applications, Nova Science Publishers, New York : 29-68
  5. Matelska D, Shabalin IG, Jabłońska J, Domagalski MJ, Kutner J, Ginalski K, Minor W (2019)
    Classification, substrate specificity and structural features of D-2-hydroxyacid dehydrogenases: 2HADH knowledgebase.
    BMC Evol. Biol. 18: 199 Times cited: 1 [Pub Med ID: 30577795] [Pub Med Central ID: PMC6303947]
  6. Mussakhmetov A, Shumilin IA, Nugmanova R, Shabalin IG, Baizhumanov T, Toibazar D, Khassenov B, Minor W, Utepbergenov D (2018)
    A transient post-translational modification of active site cysteine alters binding properties of the parkinsonism protein DJ-1.
    Biochem. Biophys. Res. Commun. 504: 328-333 Times cited: 2 [Pub Med ID: 30190129]
  7. Raczynska JE, Shabalin IG, Minor W, Wlodawer A, Jaskolski M (2018)
    A close look onto structural models and primary ligands of metallo-β-lactamases.
    Drug Resist. Updat. 40: 1-12 Times cited: 4 [Pub Med ID: 30466711] [Pub Med Central ID: PMC6260963]
  8. Handing KB, Niedzialkowska E, Shabalin IG, Kuhn ML, Zheng H, Minor W (2018)
    Characterizing metal-binding sites in proteins with X-ray crystallography.
    Nat Protoc 13: 1062-1090 Times cited: 6 [Pub Med ID: 29674755] [Pub Med Central ID: PMC6235626]
    F1000 Recommended Publication
  9. Kutner J, Shabalin IG, Matelska D, Handing KB, Gasiorowska O, Sroka P, Gorna MW, Ginalski K, Wozniak K, Minor W (2018)
    Structural, Biochemical, and Evolutionary Characterizations of Glyoxylate/Hydroxypyruvate Reductases Show Their Division into Two Distinct Subfamilies.
    Biochemistry 57: 963-977 Times cited: 2 [Pub Med ID: 29309127] [Pub Med Central ID: PMC6469932]
  10. Shabalin IG, Porebski PJ, Minor W (2018)
    Refining the macromolecular model - achieving the best agreement with the data from X-ray diffraction experiment.
    Crystallogr Rev 24: 236-262 Times cited: 4 [Pub Med ID: 30416256] [Pub Med Central ID: PMC6219471]
  11. Zheng H, Cooper DR, Porebski PJ, Shabalin IG, Handing KB, Minor W (2017)
    CheckMyMetal: a macromolecular metal-binding validation tool.
    Acta Crystallogr D Struct Biol 73: 223-233 Times cited: 45 [Pub Med ID: 28291757] [Pub Med Central ID: PMC5349434]
  12. Handing KB, Shabalin IG, Kassaar O, Khazaipoul S, Blindauer CA, Stewart AJ, Chruszcz M, Minor W (2016)
    Circulatory zinc transport is controlled by distinct interdomain sites on mammalian albumins.
    Chem Sci 7: 6635-6648 Times cited: 18 [Pub Med ID: 28567254] [Pub Med Central ID: PMC5450522]
  13. Niedzialkowska E, Gasiorowska O, Handing KB, Majorek KA, Porebski PJ, Shabalin IG, Zasadzinska E, Cymborowski M, Minor W (2016)
    Protein purification and crystallization artifacts: The tale usually not told.
    Protein Sci. 25: 720-33 Times cited: 15 [Pub Med ID: 26660914] [Pub Med Central ID: PMC4815408]
  14. Handing KB, Shabalin IG, Szlachta K, Majorek KA, Minor W (2016)
    Crystal structure of equine serum albumin in complex with cetirizine reveals a novel drug binding site.
    Mol. Immunol. 71: 143-151 Times cited: 9 [Pub Med ID: 26896718] [Pub Med Central ID: PMC4800003]
  15. Shabalin I, Dauter Z, Jaskolski M, Minor W, Wlodawer A (2015)
    Crystallography and chemistry should always go together: a cautionary tale of protein complexes with cisplatin and carboplatin.
    Acta Crystallogr D Biol Crystallogr. 71: 1965-79 Times cited: 30 [Pub Med ID: 26327386] [Pub Med Central ID: PMC4556316]
  16. Zhou D, Hansen D, Shabalin IG, Gustchina A, Vieira DF, de Brito MV, Araújo AP, Oliva ML, Wlodawer A (2015)
    Structure of BbKI, a disulfide-free plasma kallikrein inhibitor.
    Acta Crystallogr F Struct Biol Commun 71: 1055-62 [Pub Med ID: 26249699] [Pub Med Central ID: PMC4528941]
  17. Zheng H, Shabalin IG, Handing KB, Bujnicki JM, Minor W (2015)
    Magnesium-binding architectures in RNA crystal structures: validation, binding preferences, classification and motif detection.
    Nucleic Acids Res. 43: 3789-801 Times cited: 33 [Pub Med ID: 25800744] [Pub Med Central ID: PMC4402538]
  18. Zheng H, Handing KB, Zimmerman MD, Shabalin IG, Almo SC, Minor W (2015)
    X-ray crystallography over the past decade for novel drug discovery - where are we heading next?
    Expert Opin Drug Discov 10: 975-89 Times cited: 22 [Pub Med ID: 26177814] [Pub Med Central ID: PMC4655606]
  19. Shabalin IG, Porebski PJ, Cooper DR, Grabowski M, Onopriyenko O, Grimshaw S, Savchenko A, Chruszcz M, Minor W (2012)
    Structure of anabolic ornithine carbamoyltransferase from Campylobacter jejuni at 2.7 Å resolution.
    Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 68: 1018-24 Times cited: 3 [Pub Med ID: 22949186] [Pub Med Central ID: PMC3433189]