Education:
  • M.Sc. - Moscow State University (2006)
  • Ph.D. in Chemistry - A.N. Bach Institute of Biochemistry, Russian Academy of Sciences (2010)
Research Interest:
  • High-throughput structure determination and functional studies of proteins (including potential drug-targets) from pathogens causing emerging and re-emerging infectious diseases using a combination of X-ray crystallography and other biophysical methods.
  • Developing integrative techniques for highly efficient protein crystallization with a goal of maximizing the number of successful crystallizations, yet minimizing the resources used.
  • Macromolecular structure elucidation and refinement. Exploration of novel methods for crystallographic structure solution and solving "mission impossible" structures.
  • Quality assessment of crystallographic protein structures. Ligand and metal identification and judicious modeling.
  • Metal binding to protein and RNA. Developing a protocol for characterizing metal binding sites in proteins with X-ray crystallography. Developing a protocol for Mg-binding sites validation and classification in RNA crystal structures.
CV:
  • 26 peer-reviewed publications
  • Presentations at 20 conferences and workshops, including 5 invited talks
  • More than 120 protein structures in the PDB
  • Co-developed an integrative protocol for highly efficient protein crystallization. Achieved 45% success rate for a set of 95 proteins (structure determined for 46 proteins)
  • Co-developed an integrative protocol for characterizing metal-binding sites in proteins with X-ray crystallography
  • Recipient of the Best Postdoc Poster Award at 43rd Mid-Atlantic Macromolecular Crystallography Meeting (2013)
  • Four awards during graduate/undergraduate studies
  • Contributed to the development of MgRNA server https://csgid.org/csgid/metalnas/
  • Contributed to the development of CheckMyMetal server https://csgid.org/csgid/metal_sites/
  • Contributed to the development of 2HADH knowledgebase http://2hadh.bioreproducibility.org/

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Publications with the Minor Lab

  1. Czub MP, Venkataramany BS, Majorek KA, Handing KB, Porebski PJ, Beeram SR, Suh K, Woolfork AG, Hage DS, Shabalin IG, Minor W (2019)
    Testosterone meets albumin - the molecular mechanism of sex hormone transport by serum albumins.
    Chem. Sci. 10: 1607-1618 [Pub Med ID: 30842823] [Pub Med Central ID: PMC6371759]
  2. Matelska D, Shabalin IG, Jabłońska J, Domagalski MJ, Kutner J, Ginalski K, Minor W (2019)
    Classification, substrate specificity and structural features of D-2-hydroxyacid dehydrogenases: 2HADH knowledgebase.
    BMC evolutionary biology 18: 199 [Pub Med ID: 30577795] [Pub Med Central ID: PMC6303947]
  3. Kowiel M, Brzezinski D, Porebski PJ, Shabalin IG, Jaskolski M, Minor W (2018)
    Automatic recognition of ligands in electron density by machine learning.
    Bioinformatics 35: 452-461 [Pub Med ID: 30016407] [Pub Med Central ID: PMC6361236]
  4. Mussakhmetov A, Shumilin IA, Nugmanova R, Shabalin IG, Baizhumanov T, Toibazar D, Khassenov B, Minor W, Utepbergenov D (2018)
    A transient post-translational modification of active site cysteine alters binding properties of the parkinsonism protein DJ-1.
    Biochemical and biophysical research communications 504: 328-333 Times cited: 1 [Pub Med ID: 30190129]
  5. Raczynska JE, Shabalin IG, Minor W, Wlodawer A, Jaskolski M (2018)
    A close look onto structural models and primary ligands of metallo-β-lactamases.
    Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy 40: 1-12 Times cited: 1 [Pub Med ID: 30466711] [Pub Med Central ID: PMC6260963]
  6. Handing KB, Niedzialkowska E, Shabalin IG, Kuhn ML, Zheng H, Minor W (2018)
    Characterizing metal-binding sites in proteins with X-ray crystallography.
    Nat Protoc 13: 1062-1090 Times cited: 5 [Pub Med ID: 29674755] [Pub Med Central ID: PMC6235626]
    F1000 Recommended Publication
  7. Kutner J, Shabalin IG, Matelska D, Handing KB, Gasiorowska O, Sroka P, Gorna MW, Ginalski K, Wozniak K, Minor W (2018)
    Structural, Biochemical, and Evolutionary Characterizations of Glyoxylate/Hydroxypyruvate Reductases Show Their Division into Two Distinct Subfamilies.
    Biochemistry 57: 963-977 Times cited: 2 [Pub Med ID: 29309127]
  8. Shabalin IG, Porebski PJ, Minor W (2018)
    Refining the macromolecular model - achieving the best agreement with the data from X-ray diffraction experiment.
    Crystallography reviews 24: 236-262 Times cited: 2 [Pub Med ID: 30416256] [Pub Med Central ID: PMC6219471]
  9. Zheng H, Cooper DR, Porebski PJ, Shabalin IG, Handing KB, Minor W (2017)
    CheckMyMetal: a macromolecular metal-binding validation tool.
    Acta Crystallogr D Struct Biol 73: 223-233 Times cited: 40 [Pub Med ID: 28291757] [Pub Med Central ID: PMC5349434]
  10. Handing KB, Shabalin IG, Kassaar O, Khazaipoul S, Blindauer CA, Stewart AJ, Chruszcz M, Minor W (2016)
    Circulatory zinc transport is controlled by distinct interdomain sites on mammalian albumins.
    Chem. Sci. 7: 6635-6648 Times cited: 16 [Pub Med ID: 28567254] [Pub Med Central ID: PMC5450522]
  11. Handing KB, Shabalin IG, Szlachta K, Majorek KA, Minor W (2016)
    Crystal structure of equine serum albumin in complex with cetirizine reveals a novel drug binding site.
    Mol. Immunol. 71: 143-151 Times cited: 8 [Pub Med ID: 26896718] [Pub Med Central ID: PMC4800003]
  12. Niedzialkowska E, Gasiorowska O, Handing KB, Majorek KA, Porebski PJ, Shabalin IG, Zasadzinska E, Cymborowski M, Minor W (2016)
    Protein purification and crystallization artifacts: The tale usually not told.
    Protein Sci. 25: 720-33 Times cited: 13 [Pub Med ID: 26660914] [Pub Med Central ID: PMC4815408]
  13. Shabalin I, Dauter Z, Jaskolski M, Minor W, Wlodawer A (2015)
    Crystallography and chemistry should always go together: a cautionary tale of protein complexes with cisplatin and carboplatin.
    Acta Crystallogr D Biol Crystallogr. 71: 1965-79 Times cited: 29 [Pub Med ID: 26327386] [Pub Med Central ID: PMC4556316]
  14. Zhou D, Hansen D, Shabalin IG, Gustchina A, Vieira DF, de Brito MV, Araújo AP, Oliva ML, Wlodawer A (2015)
    Structure of BbKI, a disulfide-free plasma kallikrein inhibitor.
    Acta crystallographica. Section F, Structural biology communications 71: 1055-62 [Pub Med ID: 26249699] [Pub Med Central ID: PMC4528941]
  15. Zheng H, Shabalin IG, Handing KB, Bujnicki JM, Minor W (2015)
    Magnesium-binding architectures in RNA crystal structures: validation, binding preferences, classification and motif detection.
    Nucleic Acids Res. 43: 3789-801 Times cited: 33 [Pub Med ID: 25800744] [Pub Med Central ID: PMC4402538]
  16. Zheng H, Handing KB, Zimmerman MD, Shabalin IG, Almo SC, Minor W (2015)
    X-ray crystallography over the past decade for novel drug discovery - where are we heading next?
    Expert Opin Drug Discov 10: 975-89 Times cited: 21 [Pub Med ID: 26177814] [Pub Med Central ID: PMC4655606]
  17. Shabalin IG, Porebski PJ, Cooper DR, Grabowski M, Onopriyenko O, Grimshaw S, Savchenko A, Chruszcz M, Minor W (2012)
    Structure of anabolic ornithine carbamoyltransferase from Campylobacter jejuni at 2.7 Å resolution.
    Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 68: 1018-24 Times cited: 3 [Pub Med ID: 22949186] [Pub Med Central ID: PMC3433189]